Determining the efficacy of mycotoxin mitigators: in vitro vs in vivo

Commercial marketing of feed additives to prevent mycotoxicosis began about 40 years ago and is now a major industry. However, the challenge for suppliers has been proving product efficacy. In vitro methodology In vitro binding is the most commonly used methodology for investigating product efficacy. Such tests usually involve incubation of a single purified mycotoxin with a product under controlled conditions of temperature, pH and time. The major advantages of such in vitro techniques are the