Impacts of rumen fluid on gas production

Rumen fluid is commonly used to predict the nutritional value of feed. However, the collection of fresh rumen fluid is complex. This study evaluated whether refrigerated rumen fluid could be a useful alternative to fresh fluid. Gas production is a common in vitro test in many research centres and commercial laboratories as it is inexpensive, rapid and easy to reproduce with manual or automated systems. However, the collection of rumen fluid requires animals that are cannulated or intubated wi